global score

import tkinter as tk
from tkinter import*
from tkinter import ttk
from tkinter import messagebox
root=Tk()

notebook=ttk.Notebook(root)

frame1=ttk.Frame(notebook)
frame2=ttk.Frame(notebook)
frame3=ttk.Frame(notebook)

notebook.add(frame1,text="Q1A,Q1B,Q1C,Q1D")
notebook.add(frame2,text="Q2")
notebook.add(frame3,text="Q3")
        
quiz=Tk()
quiz.geometry("500x700")
quiz.title("Quiz Code")
quiz.resizable(False,False)
score=0

welcome=tk.Label(quiz,text="Welcome to Quiz")
welcome.pack()



def Inst():
    t=tk.Label(quiz,text="All you have to do is answer each question with either a 'A, B, C, D' or the actual word")
    t.pack()

def Start():    
    Question1=ttk.Label(frame1,text='Q.1.Which anime is Hinata Hyuga from?')
    Question1.grid(row=1,column=0, sticky=W)


    var=StringVar()
    Q1A=ttk.Radiobutton(frame1,text='[A] Watashi ni tenshi ga maiorita',variable=var,value='False1')
    Q1A.grid(row=2,column=0,sticky=W)

    Q1B=ttk.Radiobutton(frame1,text='[B] Oregairu',variable=var,value='False2')
    Q1B.grid(row=3,column=0,sticky=W)

    Q1C=ttk.Radiobutton(frame1,text='[C] Naruto',variable=var,value='True')
    Q1C.grid(row=4,column=0,sticky=W)

    Q1D=ttk.Radiobutton(frame1,text='[D] Overlord', variable=var, value='False3')
    Q1D.grid(row=5,column=0,sticky=W)


    submit=ttk.Button(frame1,text='Submit',command=Answer)
    submit.grid()



def Answer():
    if var.get() == 'True':
        messagebox.showinfo('Congratulations!', message='You have got the answer right!Score is {}'.format(score))
    else:
        messagebox.showinfo('Oops!', message='You have got the answer wrong!')


startButton=tk.Button(quiz,bg="green",fg="black",command=Start,text="Start")
startButton.pack()
instr=tk.Button(quiz,bg="grey",fg="black",command=Inst,text="User Instructions")
instr.pack()
end=tk.Button(quiz,text="Exit",command=quiz.destroy)
end.pack()

quiz.mainloop()

Error:
Exception in Tkinter callback
Traceback (most recent call last):
  File "C:\Users\Ramaz\AppData\Local\Programs\Python\Python39\lib\tkinter\__init__.py", line 1884, in __call__
    return self.func(*args)
  File "C:\Users\Ramaz\OneDrive\Documents\Mustafa Work\Quiz code.py", line 35, in Start
    Question1=ttk.Label(frame1,text='Q.1.Which anime is Hinata Hyuga from?')
  File "C:\Users\Ramaz\AppData\Local\Programs\Python\Python39\lib\tkinter\ttk.py", line 759, in __init__
    Widget.__init__(self, master, "ttk::label", kw)
  File "C:\Users\Ramaz\AppData\Local\Programs\Python\Python39\lib\tkinter\ttk.py", line 557, in __init__
    tkinter.Widget.__init__(self, master, widgetname, kw=kw)
  File "C:\Users\Ramaz\AppData\Local\Programs\Python\Python39\lib\tkinter\__init__.py", line 2568, in __init__
    self.tk.call(
_tkinter.TclError: NULL main window