my code here

import Tkinter as tk
from Tkinter import *
import ttk

root = tk.Tk()
root.title("GUI")
root.geometry("300x200")


#####################___________ Profile-1 ___________#####################    
def Profile_1():
   print('Profile 1 selected')

#####################___________ Profile-2 ___________#####################  
def Profile_2():
   print('Profile 2 selected')

#####################___________ Profile-3 ___________#####################  
def Profile_3():
   print('Profile 3 selected')
#####################___________ Profile-4 ___________#####################          
def Profile_4():
   print('profile 4 selected')


def serial_port():
   global var
   print('It comes in to push button menu')
   x = Profile_sel_1.selection_get()
   print(x)
   
var = IntVar()
#Button to show selected profile to assign FTW
Profile_sel_1=Radiobutton(root, text='Profile Selection_1',variable =var, value =1,command = Profile_1,width = 20)
Profile_sel_2=Radiobutton(root, text='Profile Selection_2',variable =var, value =2,command = Profile_2,width = 20)
Profile_sel_3=Radiobutton(root, text='Profile Selection_3',variable =var, value =3,command = Profile_3,width = 20)
Profile_sel_4=Radiobutton(root, text='Profile Selection_4',variable =var, value =4,command = Profile_4,width = 20)
#Button to show entered reg values and data in it
ser_port_btn=Button(root, text='ENTER',command = serial_port,relief="flat",background="#ccc",width=20)
Profile_sel_1.grid(row=1,column = 5)
Profile_sel_2.grid(row=2,column = 5)
Profile_sel_3.grid(row=3,column = 5)
Profile_sel_4.grid(row=4,column = 5)
ser_port_btn.grid(row = 7, column = 3)

root.mainloop()

# boiler plate code for python2/3 compatibility
# i don't like to run python2.7
from __future__ import print_function
from functools import partial
try:
    import Tkinter as tk
    from Tkinter import *
    import ttk
except ModuleNotFoundError:
    import tkinter as tk
    from tkinter import *  
    from tkinter import ttk
# you don't need it


def serial_port(var):
    selection = var.get()
    # get the value out of var, which has been defined in main()
    print('Pushed the button!')
    print('var has value', selection)
    # to save space
    text_dict = {
        0: 'Nothing selected!', 
        1: 'Profile 1 selected',
        2: 'Profile 2 selected',
        3: 'Profile 3 selected',
        4: 'profile 4 selected'
        }
    # you can also put functions as a value to the text_dict
    # and call them after you got the value
    # d = {1: function}
    # d[1]('do_something')
    # just as a hint
    text_to_print = text_dict[selection]
    print(text_to_print)


def main():
    root = tk.Tk()
    root.title("GUI")
    root.geometry("300x200")    
    var = IntVar()
    #Button to show selected profile to assign FTW
    profile_sel_1=Radiobutton(root, text='Profile Selection_1', variable=var, value=1, width=20)
    # just removed command
    # you can preselect a Radiobutton with:
    # profile_sel_1.select()
    # or you can use the variable var, to select the Radiobutton
    # var.set(1)
    profile_sel_2=Radiobutton(root, text='Profile Selection_2', variable=var, value=2, width=20)
    profile_sel_3=Radiobutton(root, text='Profile Selection_3', variable=var, value=3, width=20)
    profile_sel_4=Radiobutton(root, text='Profile Selection_4', variable=var, value=4, width=20)
    # Button to show entered reg values and data in it
    ser_port_btn=Button(root, text='ENTER', command=partial(serial_port, var), relief="flat", background="#ccc", width=20)
    # or use (..., command=lambda: serial_port(var), ...)
    profile_sel_1.grid(row=1, column=5)
    profile_sel_2.grid(row=2, column=5)
    profile_sel_3.grid(row=3, column=5)
    profile_sel_4.grid(row=4, column=5)
    ser_port_btn.grid(row=7, column=3)
    root.mainloop()


if __name__ == '__main__':
    main()