sub-folders in folders from text line

[ndc85430]

I don't understand the question. You can use os.mkdir to create directories. There's also os.makedirs if you want to give a path and create all intermediate directories to the leaf (i.e. like mkdir -p on the command line would do).

Also, no, you don't need to close the file - using with takes care of that for you (go and read about context managers if you're interested).