[Tkinter] Sections of the code causes the GUI to freeze. What should I do?

[DeaD_EyE]

if pyautogui.locateCenterOnScreen("loot_icon.png", confidence = 0.9):
    ...

pyautogui.locateCenterOnScreen could return None or a tuple with two int.

The if statement asks the object for its boolean. bool(None) evaluates to False.
An empty list, tuple, dict, set, str, bytes, etc. evaluates to False. If they contain elements, then the bool() call returns True.

The result that you want is a non-empty Tuple, so the approach not to check for None, instead check for truthiness, is better.