Python Code Generator

[MTVDNA]

Cool project!

I wrote this example for you that 'transfers' mastermix to a series of wells as you wanted. Note that I used a dummy transfer function to generate some output. You'll need to adjust this of course.

The idea is: first you make a list of sources for both your mastermix and cDNA. Then, for each mastermix, you must make a list of target wells to transfer them to. Then you make the transfer.

# dummy transfer function, just to test
def transfer(p_amount, p_source, p_target):
 print("Transfering {:>5}ul from {} to {}".format(p_amount, p_source, p_target))


# list of sources of mastermix and cDNA
mastermix_sources = ['A1', 'A2']
cDNA_sources = ['B1', 'B2', 'B3']


# loop through each source of mastermixes
for i, mm in enumerate(mastermix_sources):
 
 # make a list of all targets for this mastermix
 targets =list("{}{}".format(chr(65+i), j+1) for j in range(len(cDNA_sources)))
 
 # transfer mastermixes to wells
 transfer(
           10, 
           mm,
           targets
         )

Which gives me the output:

Output:
Transfering    10ul from A1 to ['A1', 'A2', 'A3']
Transfering    10ul from A2 to ['B1', 'B2', 'B3']

Now this line is a bit complicated, so let me explain:

targets = list( "{}{}".format(chr(65+i), j+1) for j in range(len(cDNA_sources)) )

It generates a list of targets like this:

• for j in range(len(cDNA_sources))
  loops j through numbers 0 up to the amount of cDNA sources you have
  
• "{}{}".format( chr(65+i), j+1)
  format replaces {} in the string with the arguments given:
  • chr(65+i)
    chr returns an ASCII character, chr(65) is A, chr(66) is B, etc. i is the index of this mastermix which we have because we used enumerate
    
  • j comes from the for loop. We want the first target to be A1, and j starts at 0, so we add 1
    
• list makes it into a list (obviously :p)
  

If anything else is unclear don't hesitate to ask.

Now try and see if you can adapt this example so you can generate the transfers from the cDNA_sources to the wells as well.