Nested Conditionals

[nilamo]

birds = ("Pigeon, Eagle, Parrot, Chicken, Turkey, Macaw, Duck, Geese")

Ok, so that's not a list, or tuple, of birds. It's a single string.
If you fix that, the rest of your code might work.

if last_guess.title() in birds == False:

This is a bit of a nitpick, but there's no reason to have the == False there. The in operator already returns True/False, so it's redundant, and makes the code a little harder to read.