tkinter GUI, problem running seperate files

[fishglue]

Hello. I'm fairly new to Python.

If I run the program from my editor it works fine, every .py file opens when buttons is pressed and does what it's supposed to. Once I try to double click the main program or run it with a hotkey, and click the button that opens the .py file, nothing happens. In this case the button labeled "test" inside class StartPage.

What I want to happen: The program has to open seperate .py files when a button is clicked, when the program is started with a hotkey.

I have tried:
-subprocess.Popen
-subprocess.call
-os
-change to .pyw
-pythonpath
-specified pathing to .py file

I run python 3.7
Windows 10 MSC v.1915 64 bit (AMD64)

The code is 400 lines, so I made it a bit shorter:

import tkinter as tk
from tkinter import ttk
import subprocess
import time




class MainClass(tk.Tk):

    def __init__(self, *args, **kwargs):
        
        tk.Tk.__init__(self, *args, **kwargs)
        container = tk.Frame(self)
        self.geometry("1000x700+300+60")
        container.pack(side="top", fill="both", expand = True)

        container.grid_rowconfigure(0, weight=1)
        container.grid_columnconfigure(0, weight=1)

        self.frames = {}

        for F in (StartPage, PageOne, PageTwo, PageThree, PageFour, PageFourSubOne, PageFive, PageSix):

            frame = F(container, self)

            self.frames[F] = frame

            frame.grid(row=0, column=0, sticky="nsew")

        self.show_frame(StartPage)

    def show_frame(self, cont):

        frame = self.frames[cont]
        frame.tkraise()

        
class StartPage(tk.Frame):

    def __init__(self, parent, controller):
        tk.Frame.__init__(self,parent)
        
        label = tk.Label(self, text="Hovedmeny", font=("arial", 20), fg="Blue")
        label.pack(pady=10,padx=10)
        

        def test():
            subprocess.call("autoguitest2.py", shell=True)
            controller.show_frame(StartPage)

#input('1: press enter to continue') (Coudn't get this working)

        testbase = ttk.Button(self, text="Test", command=test)
        testbase.pack()

class PageOne(tk.Frame):

    def __init__(self, parent, controller):
        tk.Frame.__init__(self, parent)
        label = tk.Label(self, text="CO2", font=LARGE_FONT)
        label.pack(pady=10,padx=10)
        
        style = ttk.Style()
        style.configure("tilbake.TButton", width=20, height=2, font=('arial', 12, "bold"), bg="Blue")
        
        hovedmeny = ttk.Button(self, text="Tilbake til hovedmenyen", style="tilbake.TButton",
                            command=lambda: controller.show_frame(StartPage))
        
        hovedmeny.pack()
        
        label = tk.Label(self, text="Label", font=('arial', 15, "bold"), fg="Red")
        label.pack(pady=10,padx=10)

        def trekilo_ordre():
            subprocess.call("CO2.py", shell=True)
            controller.show_frame(StartPage)
            
            
        style.configure("3kg.TButton", width=40, height=2, font=("arial", 12, "bold"))

        trekilo = ttk.Button(self, text="CO2 3kg", style="3kg.TButton", command=trekilo_ordre)


app = MainClass()
app.mainloop()