For Loop with List Comprehension

[buran]

print(item, sep=' ') - you pass just one object as first argument to print function, a tuple with n elements. It prints a tuple (i.e. incl brackets and elements separated by comma - that is str representation of a tuple). In this case sep does not come into play - there is one argument.

print(*item, sep=' ') - note the asterisks. This is iterable unpacking. you unpack the iterable (i.e. the tuple). print function gets n positional elements, not single tuple. Then sep comes into play and you get the output you expect.