[PyQt] Button clicked not working

[catlessness]

    os.system('python -m PyQt5.uic.pyuic PI_GUI_App_Map.ui -o PI_GUI_App_Map.py')

Do that once (in a terminal)and remove this line.

Without knowing the code of PI_GUI_App_Map and PI_GUI_Funcc and count.txt it is difficult to help.

Thanks for replying.

yes, I have tried that (without os.system()).
PI_GUI_App_Map.py is transformed from the ui file.
PI_GUI_Func includes a class to start up a child thread.
count.txt is a file created by another equipment, which is monitored by this program to see when it changes (basically just 1, 2, 3...).
The py files are attached.

Many thanks